Need help in planning a study on neonatal septicemia!!

[selvanr4]

We have 60 % non culture proven sepsis in newborns. I doubt that many of them might have viral sepsis. None of the studies from india haveidentified the viral etiology.Hence our aim is to organise a study to find out the other etiologies of neonal sepsis.I need experts help in formulating, diagnosing and proving this.
I request the advice from the learned .
Thanks
dr.r.selvan
Erode, India

[Stefan Johansson]

Have you yet decided what viruses that you may want to diagnose?

[selvanr4]

My list has 1. Herpes simplex 2. CMV 3. Respiratory viruses 4.entero virus 5. parvo virus. I would like to look out for atypical organism. Please suggest me the best diagnostic methods.
selvan.dr.

[Stefan Johansson]

I'd think you could do PCR (on liqour) for at least #1, #2, #4 and #5.
one of my own study from my thesis may give some hints on diagnostics : http://www.ncbi.nlm.nih.gov/pubmed/18930808

My suggestion is that you involve your lab and discuss diagnostics with them too. After all, someone will need to do the analyses!

Wish you all best of luck!

[selvanr4]

Thanks for the suggestions.
I will update the developements
dr.r.selvan